Instructions for Microscopic Examination of Spores

Recommended Tools:

• Spore print or spore syringe

• Microscope (40x – 1000x)

• Pipette or syringe

• Inoculation loop

• Water

• Object slide and cover slip

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General Procedures:

The form, colour, and size of spores are distinctive traits used for mushroom classification. To prepare your sample, start by placing a drop of water on a clean object slide. Use the inoculation loop to stir in a few spores, then cover it with a cover slip. Place the slide under the microscope to observe the spores.

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Alternatively, you can place a drop of spore solution from a spore syringe directly onto the object slide and cover it with a slip. If using a spore syringe, we recommend shaking it well to ensure the spores are evenly dispersed in the solution.

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Ensure that the microscope is positioned on a flat and stable surface for accurate viewing.